University of Pittsburgh researchers have developed a new device that aims to improve microdialysis experiments by using electroosmosis to better control substance concentrations in the infused tissue.
Description
Commonly, microdialysis probes are inserted into tissue to capture solutes in the extracellular space (ECS) based on passive diffusion of the solutes from the ECS to the probe. Often, substances such as drugs may be introduced into the ECS by passive diffusion from the probe. With the current paradigm, there is the possibility of tissue trauma and lack of control over the concentration of infused substances. This new device uses electroosmotic flow through a very small capillary to infuse substances into the tissue, through the tissue and into a second very small capillary in the tissue. The flow passes by the microdialysis probe which is external to the tissue being investigated. The device comprises the two small capillaries and a vessel for the microdialysis probe. This design minimizes trauma, improves control over concentrations, and allows for measurements of reaction rates in the extracellular space.
Applications
• Microdialysis experiments
• Drug delivery
Advantages
Currently, some microdialysis experiments may need a second cannula for substance infusion, thus compromising precision and efficiency. This innovation is equally capable of (1) measuring the natural concentration of, e.g., a neurotransmitter or (2) measuring the effect of an inhibitor, antagonist, agonist, on the concentration of a sought-for species. It uses an electrical current to control the flow rate of infused substances in tissue, providing a more precise and less complicated system. This novel device places the (relatively large) microdialysis probe outside of the tissue which decreases the potential for tissue trauma.
Invention Readiness
This invention is currently in the in vivo data stage of development.
IP Status
https://patents.google.com/patent/WO2023044070A2
