This system employs cyclical pressure changes within an airtight chamber to remove cellular components while retaining the integrity of the extracellular matrix. Tissue samples are immersed in decellularization solutions that may include surfactants, salts, sugars, enzymes, or acids, and are subjected to computer-controlled pressure cycles. Parameters such as adjustable cycle frequency, variable pressure change magnitudes, precise ramp rates, and tailored duty cycles ensure that cellular material is efficiently removed while preserving the native tissue structure and mechanical properties. This method has been successfully applied to a range of tissues including aorta, vocal fold, trachea, and dermis, catering to advanced regenerative medicine and tissue engineering applications.
Description
What sets this technology apart is its precision and adaptability compared to conventional methods. The controlled cycling of pressure minimizes physical disruptions often associated with traditional agitation techniques, thus maintaining superior extracellular matrix properties. The ability to fine-tune process parameters—such as the rate and magnitude of pressure changes and the duration of pressure holds—ensures optimal decellularization tailored to each tissue type. This results in a more consistent and mechanically robust end product, making it a uniquely effective and efficient approach for applications requiring high-quality tissue scaffolds.
Applications
Regenerative medicine scaffold production
Artificial organ manufacturing
Tissue graft engineering
Biocompatible implant development
Cell culture ECM creation
Advantages
Enhanced removal of cellular components compared to conventional methods
Superior preservation of the native extracellular matrix structure and mechanical properties
Automated, precise control of pressure parameters for consistent decellularization
Applicability to a wide range of tissue types, including both delicate and fibrous tissues
Improved speed and overall efficacy of the decellularization process
IP Status
https://patents.google.com/patent/US11185611B2
