Biomimetic fibrous hydrogel scaffolds are created through the controlled self-assembly of oppositely charged polysaccharides, mimicking the natural extracellular matrix of bone. Using a microfluidic system with dual syringe pumps and an extended needle, polymer solutions align to form anisotropic fibers with nanoscale periodic bands and larger fibril bundles. This method enables precise mineral sequestration from simulated body fluid, resulting in bone-like apatite deposits. The system is adaptable, with variants formulated from different polysaccharide pairs and further modifiable by incorporating conductive graphene or bioactive peptides to enhance mechanical properties, cellular alignment, and drug delivery functions.
Description
This technology is differentiated by its ability to replicate the hierarchical structure of natural bone without relying on cytotoxic chemical crosslinkers. Its electrostatic assembly mimics the intrinsic charge interactions and collagen architecture found in tissue, leading to superior biomineralization and integration. Extensive analytical methods—including microscopy, spectroscopy, and mechanical testing—confirm its ordered mineral deposition and structural resilience. The injectable, biodegradable, and cost-effective nature of these scaffolds provides a versatile platform for regenerative therapies, targeted drug delivery, and engineered tissue applications, setting it apart as a transformative approach in tissue engineering.
Applications
- Bone tissue regeneration scaffolds
- Injectable regenerative biomaterials
- Controlled drug delivery system
- Vascular graft tissue engineering
Advantages
- Mimics natural bone extracellular matrix for enhanced tissue integration.
- Enables controlled, consistent nanoscale fibrous alignment and hierarchical structure.
- Uses electrostatic self-assembly to avoid cytotoxic chemical crosslinkers, ensuring biocompatibility.
- Offers versatile polymer combinations to tailor mineralization and mechanical properties.
- Provides an injectable, deformation-resistant scaffold with a long shelf life for practical clinical use.
- Supports modifications for cell alignment, drug delivery, and broader tissue engineering applications.
IP Status
https://patents.google.com/patent/US11179502B2
