These tools stand out by combining molecular, immunohistochemical, and serological approaches that are finely tuned to MCV’s biology. The exon-2–specific antibody does not cross-react with T antigens from other polyomaviruses, ensuring unambiguous tumor identification. The qPCR method not only detects presence but also measures viral burden in situ, while the VLP-ELISA and reporter neutralization assay provide both binding and neutralization data, enabling a comprehensive assessment of exposure and immunity with quantitative precision unmatched by existing assays.
Description
Using digital transcriptome subtraction on a Merkel cell carcinoma tumor, the inventors have identified and isolated a cDNA similar to but distinct from an oncogene from a known class of viruses that cause tumors in animals. This virus is now called Merkel Cell Virus (MCV). Using this sequence, they have isolated the remaining sequence of the virus genome. They have identified this viral nucleic acid in 6 of 8 MCC tumors but none of >50 control human tissue nucleic acids. Additional tissue panel searches have revealed that MCV is rarely found in other malignant and nonmalignant tissues but may also be the cause of additional tumors and human diseases.
Applications
Merkel cell carcinoma diagnostics
MCV viral load quantification
Anti-MCV serology ELISA kits
MCV neutralization assay kits
VLP antigen production services
Advantages
Enables highly specific immunohistochemical detection of MCV large T antigen in Merkel cell carcinoma, improving diagnostic accuracy
Provides a quantitative PCR assay to measure viral integration and copy number in tumor samples, allowing precise assessment of tumor viral burden
Uses virus‐like particle–based ELISA to detect anti‐MCV antibodies, facilitating seroepidemiological studies and monitoring of immune exposure
Offers a reporter‐based neutralization assay with enhanced sensitivity for functional antibody response measurement, supporting vaccine and therapeutic evaluation
IP Status
https://patents.google.com/patent/US9701734B2
