Engineered Cell Line Platform for Studying Herpes Simplex Virus Immediate-Early Protein Functions

The FO6 cell line is derived by transfecting roughly 1 million E26 cells with specific plasmids that introduce key herpes simplex virus components, such as ICP4, ICP0, and ICP27. The process involves the use of pW3DHS8 along with pSV2Hyg, the latter of which carries a hygromycin resistance gene under the simian virus 40 early promoter. This technology offers a robust platform by enabling the controlled expression of viral proteins in a consistent cellular background.

Description

What sets this technology apart is its precise genetic engineering that opens new avenues for the study of viral gene regulation and cell survival mechanisms. Its construction ensures reproducibility and reliability, while the documented methodology and regulatory compliance affirm its scientific rigor. The careful integration of resistance markers and viral protein expression allows researchers to dissect intricate virus-host interactions, thereby providing a specialized tool that stands out from more traditional research models.

Applications

- Antiviral drug screening
- Vaccine candidate evaluation
- Herpes virus research

Advantages

- Provides a reliable cellular model expressing key herpes simplex virus proteins for in-depth viral function studies.
- Enables reproducible research through its deposition at ATCC, ensuring broad availability for future studies.
- Facilitates analysis of viral gene regulation and host interactions due to its engineered genetic attributes.
- Supports robust selection using a hygromycin resistance marker, enhancing experimental consistency.