Description
To determine the role that gastrin-releasing peptide receptor (GRPR) contributes to lung tumorigenesis, we have generated transgenic mice overexpressing human GRPR in the airway epithelia of FVB mice by using clara cell secreting protein (CCSP) promoter. Overexpression of GRPR was verified in the transgenic mouse lung tissue by RT-PCR. Mouse lungs were inflated and fixed with formalin, paraffin embedded, and sectioned. Histological assessment showed that GRPR transgenic mouse lungs exhibited tortured and wide bifurcations of the airway as well as variant degrees of proliferation of bronchiolar epithelial cells compared to their wild-type littermates. Immunohistochemistry against CCSP showed clustered clara cells in the bronchiolar epithelium of transgenic compared to the interspersed clara cells in the wildtype mice.
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