Inducible Mouse Models of Tim-3 Over-Expression and Deficiency
This technology employs genetically engineered mouse models that use Cre-loxP systems for precise spatial and temporal regulation of the transmembrane protein Tim-3. One model facilitates controlled over-expression of Tim-3, while the other enables its conditional knockout, allowing researchers to activate or remove Tim-3 in specific cell types at desired time points. This targeted approach minimizes developmental compensation and offers detailed insights into immune regulation related to T cell function, cancer, autoimmunity, and infectious diseases.
Description
What differentiates this technology is its inducible nature, which offers unparalleled control over gene expression compared to traditional transgenic or constitutive knockout models. By allowing modulation of Tim-3 expression in adult mice with minimal developmental interference, it enables more accurate studies of disease mechanisms and immune dynamics. The system’s cell-specific targeting and time-sensitive activation provide researchers with a powerful tool to parse the intricacies of immune responses and pathological conditions. This level of control not only enhances experimental precision but also broadens potential applications in cancer immunotherapy, immune tolerance studies, and infectious disease research.Applications
- Cancer immunotherapy testing- Autoimmune disease modeling
- Infectious disease research
- T cell function analysis
Advantages
- Enables precise, cell-specific, and temporally controlled manipulation of Tim-3 expression.- Minimizes developmental compensatory effects by allowing gene modulation in adult mice.
- Facilitates detailed studies of immune regulation, T cell function, and related disease mechanisms.
- Provides a versatile platform to explore the roles of Tim-3 in cancer immunology, autoimmunity, and infectious diseases.