University of Pittsburgh researchers have developed a novel controlled release platform utilizing nitro-oleic acid (NO2-OA) to enhance regional angiogenesis in abdominal wall repair. This innovative technology combines micro-fibrous poly(ester carbonate)urethane urea (PECUU) scaffolds with a hydrogel derived from decellularized porcine dermis and poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with NO2-OA. The platform aims to improve vascularization and tissue integration in abdominal wall defect repairs, addressing the limitations of current prosthetic materials.
Description
The technology involves the integration of NO2-OA-loaded PLGA microspheres into PECUU scaffolds combined with a dermal extracellular matrix (ECM) hydrogel. This biohybrid scaffold design promotes controlled release of NO2-OA, which induces angiogenesis through the activation of hypoxia-inducible factor 1-alpha (HIF-1α). The scaffold's structure and mechanics can be tailored by modifying the polymeric matrix chemistry, electrospinning parameters, and microparticle morphology, providing a versatile platform for tissue engineering applications.
Applications
• Abdominal wall defect repair
• Cardiovascular tissue repair and augmentation
• Tissue engineering and regenerative medicine
• Controlled release of bioactive compounds
Advantages
The controlled release of NO2-OA from the scaffold promotes vascularization, leading to better tissue remodeling and mechanical strength. The platform's versatility allows for customization of scaffold properties to meet specific clinical needs, making it a promising solution for various tissue engineering applications.
Invention Readiness
The technology is currently at the in vivo data stage. Initial experiments have demonstrated that NO2-OA release significantly enhances regional angiogenesis, increases wall thickness, and reduces fibrotic response in a rat abdominal wall defect model. The scaffold's structure and mechanics were characterized, showing successful integration of PECUU fibers, dermal ECM gel, and NO2-OA-loaded PLGA microparticles. Histological assessments confirmed higher cellular infiltration and reduced fibrous encapsulation in the treated groups. Blood vessel morphology and spatial distribution were analyzed, revealing improved neovascularization in the scaffold-implanted regions.
IP Status
https://patents.google.com/patent/WO2019100021A1
