Description
University of Pittsburgh researchers have developed an invention, related to the field of imaging intracellular proteins, which uses fluorescent probes capable of changing color over time to identify and track the synthesis, trafficking, and secretion of intracellular proteins. One such probe, a monomeric multifluorescent protein known as a mcTimer probe, has proven useful as a biosensor for secretory proteins. In particular, a monomeric multifluorescent protein is inserted into the C-linker portion of the insulin prepropeptide as a marker for normal insulin responses to glucose in cultured donor beta cells. After expression, the mcTimer probe sequentially changes from green to red over a twenty-four hour period.
IP Status
https://patents.google.com/patent/WO2009148549A2