This technology employs a modified splitFAST system that incorporates TERT and STAT5 sequences into plasmids encoding complementary halves of a fluorescent FAST molecule. When TERT and STAT5 interact within cells, the respective FAST fragments reassemble into an active fluorescent complex, enabling real-time visualization of protein-protein interactions with both spatial and temporal resolution. The system is supported by in vitro experimental data and utilizes established molecular biology methods, making it a practical tool for researchers seeking to monitor dynamic cellular processes.
Description
This approach is differentiated by its targeted design that builds upon traditional splitFAST methodologies while introducing specific sequences for TERT and STAT5. The customization of plasmids to create a binary, interaction-dependent fluorescence system offers high specificity, ensuring that a fluorescent signal is only generated upon true protein interaction. Supported by robust experimental validation and funding from esteemed institutions, the technology provides a reliable framework for detailed study of cellular regulatory mechanisms, setting it apart from more generalized protein interaction assays.
Applications
- Real-time interaction assay
- Drug discovery platform
- Cancer biomarker analysis
- Cell signaling research
Advantages
- Real-time visualization of protein-protein interactions within living cells.
- Enables simultaneous monitoring of spatial and temporal dynamics.
- Specifically tailored for studying TERT-STAT5 interactions, aiding in the understanding of key regulatory mechanisms.
- Builds on and enhances established splitFAST technology with validated in vitro efficacy.
IP Status
Research Tool
