Modular, Lightweight Tissue Stretcher for High-Resolution Live Cell Imaging
University of Pittsburgh researchers have developed an innovative tissue stretcher system capable of applying more than 100% mechanical strain on living tissues, which can be live imaged using high-resolution confocal microscopy. This modular and lightweight system consists of a cassette, motorized actuators, and a customized microscope stage, enabling precise and cost-effective live cell imaging for multiple samples. The system's design facilitates easy sample exchange and integration with advanced microscopy techniques, making it a valuable tool for developmental mechanobiology and other biomedical research fields.
Description
The tissue stretcher system is designed to apply significant mechanical strain to living tissues while allowing for high-resolution live cell imaging. The system comprises three main components: a 3D-printed cassette, motorized actuators, and a customized microscope stage. The cassette holds the tissue samples and can be easily swapped in and out. The motorized actuators stretch the cassette, and the entire setup is compatible with inverted brightfield or confocal light microscopes. The lightweight design (less than 200 g) ensures compatibility with precise z-stage controllers, enabling detailed imaging within the 150-micrometer working distance of the microscope objective.Applications
• Developmental mechanobiology research• High-resolution live cell imaging
• Biomedical research involving tissue mechanics