This rabbit monoclonal antibody selectively targets human RIF1 only when phosphorylated at serine-2205 and mouse RIF1 only when phosphorylated at serine-2153. Its comprehensive characterization includes complete nucleotide and amino acid sequences for both heavy and light chains with delineated signal peptides, framework regions, and complementarity determining regions. This targeting is critical for detecting phosphorylation events that disrupt the interaction between RIF1 and protein phosphatase 1, offering precise insights into events governing DNA replication origin licensing and associated kinase signaling pathways.
Description
What distinguishes this technology is its extraordinary specificity and detailed sequence information, which ensure reproducibility and enhanced interpretation of phosphorylation-driven regulatory mechanisms. Unlike broader detection methods, its ability to differentiate species-specific RIF1 modifications at precise serine residues positions it as a robust tool for in vitro studies. This level of refined detection not only contributes to a deeper molecular understanding but also provides a foundation for potential translational applications in research on replication origin regulation and kinase signaling modulation.
Applications
- Phospho-RIF1 detection assay
- DNA replication investigation tool
- Kinase inhibitor screening assay
Advantages
- High specificity: Detects human RIF1 only when phosphorylated at serine-2205 and mouse RIF1 only when phosphorylated at serine-2153, ensuring precise identification of phosphorylation events.
- Enhanced mechanistic insights: Facilitates in vitro studies of the role of RIF1 phosphorylation in disrupting its association with protein phosphatase 1 (PP1) and regulating DNA replication origins.
- Comprehensive characterization: Provides complete nucleotide and amino acid sequences of the antibody’s heavy and light chains, supporting reproducibility and further development.
Research advancement: Offers a valuable tool for studying kinase signaling pathways and understanding DNA replication control, with potential implications in cancer biology and therapeutic research.
IP Status
Research Tool
