PNA-Linked Electrochemical Biosensor Platform for Quantitative Analyte Detection
Investigators at the University of Pittsburgh have developed a novel biosensor utilizing metallized peptides which serve as conduits of electronic signals that bridge between a redox enzyme and a carbon-nanotube electrode. This invention optimizes contact and geometry between the redox enzyme and the carbon nanotube electrode array allowing for superior analyte sensing. This technology has been successful in detecting Prostate Specific Antigen (PSA). The electrochemical detection of PSA was carried out using cyclic voltammetry and exhibited low detection limit of 0.1 ng/mL.
Description
This approach stands out due to its modularity and enhanced electron-transfer efficiency. The chemically tunable PNA spacer provides precise spatial control and robust attachment of biological elements, while metal coordination or nanoparticle decoration boosts signal transduction. Compared with conventional immunoassays, it achieves higher sensitivity at clinically relevant concentrations, reduced assay times and lower cost. Its compatibility with nanoelectrodes and point-of-care devices permits rapid electrochemical detection in compact formats. By combining flexible assay design with improved conductivity, the platform offers a versatile, scalable solution for fast, sensitive biomarker detection beyond PSA.Applications
- Point-of-care PSA testing- Portable cancer biomarker detection
- Continuous glucose monitoring devices
- Environmental toxin detection sensors
- Food allergen biosensor kits
Advantages
- High sensitivity detection of PSA at clinically relevant concentrations- Modular PNA‐linked design easily adapted to other target analytes
- Enhanced electron transfer via metal‐coordinated PNA spacers or gold nanoparticles
- Rapid, real‐time electrochemical readout suitable for point-of-care use
- Lower cost and simplified workflow compared to conventional laboratory assays
- Flexible assay formats (displacement and sandwich) for diverse diagnostic needs