The engineered transgenesis plasmid is designed to express Enhanced Green Fluorescent Protein (EGFP) in zebrafish melanophores by leveraging the fugu Tyrp1 promoter. It incorporates the Tol2 transposon system to achieve efficient and stable genomic integration, ensuring precise cell-specific expression in melanophores, which are neural crest-derived pigment cells. This tool facilitates real-time visualization of melanophore development, migration, and cellular function in vivo, and offers a unique opportunity for related studies on the retinal pigment epithelium given the shared developmental lineage between these cell types.
Description
This tool is differentiated by its precise targeting capability and the incorporation of advanced genetic elements like the fugu Tyrp1 promoter and the Tol2 transposon system, which together ensure robust, cell-specific expression. It stands out in the field due to its established specificity for melanophore tracking, ease of adaptation for diverse research applications, and strong validation through extensive publication citations. Moreover, its collaborative development and broad distribution further highlight its potential impact and versatility as a cutting-edge research tool in developmental biology and pigment cell research.
Applications
- Zebrafish imaging research reagent
- Melanophore assay development kits
- Neural crest cell tracking platform
- Pigmentation disorder drug screening
Advantages
- Cell-specific EGFP expression enables precise visualization and tracking of zebrafish melanophores in vivo.
- The Tol2 transposon system ensures efficient and stable genomic integration for long-term studies.
- Real-time monitoring of melanophore development, migration, and function facilitates detailed biological insights.
- The tool’s versatility allows for adaptation to express other genes of interest in specific cell types.
- It supports related studies on retinal pigment epithelium cells due to their shared developmental origins with melanophores.
IP Status
Research Tool
