Cell surface expression enhancing peptides (CSEEPs) are short peptide sequences derived from a GPCR’s N-terminal region that are genetically fused to target GPCRs to boost their transport to the cell surface. This modification results in higher surface and overall protein expression, which is crucial for applications such as structure-based drug design, biophysical analysis, and antibody development. The technology works by directly modifying the GPCR DNA sequence, enabling researchers to overcome challenges associated with low expression levels of these receptors.
Description
The approach is differentiated by its unique fusion method that employs distinct peptides rather than relying on viral-derived sequences. This strategy significantly improves receptor trafficking, making it applicable across diverse GPCR families regardless of their specific ligands or functions. Its ability to enhance protein availability not only accelerates research in structural studies and drug discovery but also offers a novel solution compared to conventional optimization of expression systems. This innovative method provides a critical tool for scientists seeking to expand the utility of GPCR analysis and therapeutic development.
Applications
- Structure-based drug design
- GPCR antibody development
- High-yield GPCR expression
- Receptor biophysical analysis
Advantages
- Significantly increased GPCR surface and overall protein expression levels.
- Enhanced receptor trafficking to the cell membrane.
- Greater availability of GPCR proteins for structure-based drug design and biophysical studies.
- Facilitates efficient antibody development targeting GPCRs.
- Broad applicability across diverse GPCR families, overcoming traditional expression challenges.
