Integrated Neural Imaging and Stimulation System for Real-Time Visualization and Control of Neural Activity

This innovative platform integrates advanced optical stimulation with neural imaging by combining a genetically encoded calcium indicator (GCaMP6) and a red-shifted opsin (ReaChR). It utilizes an InVista miniature microscope that employs blue LED light (470nm) to capture real-time cellular calcium dynamics through a 500nm GRIN lens. At the same time, a 593nm laser light is delivered through the same optical pathway to trigger neural activation. This dual-function configuration provides concurrent monitoring and targeted stimulation of neurons in vivo, enabling precise experiments to explore cellular behaviors and neural circuit dynamics.

Description

The technology stands out due to its unique integration of pre-existing components into a single, streamlined system that allows simultaneous imaging and stimulation—something not available in earlier solutions. By leveraging a shared optical conduit and combining distinct wavelengths for separate tasks, it minimizes complexity and interference while enhancing spatial precision and temporal control. This approach offers researchers a powerful tool for dissecting neural function and addressing intricate questions in neuroscience research.

Applications

- In vivo neural imaging platform
- Neural circuit function analysis
- Simultaneous imaging and stimulation
- Neurological disorder research tool

Advantages

- Enables simultaneous real-time imaging and optical stimulation of neurons.
- Provides precise control over neural activity while monitoring calcium dynamics.
- Integrates advanced optogenetic tools with miniature microscopy for in vivo applications.
- Facilitates sophisticated neuroscience experiments to study neural circuits and disorders.

IP Status

Research Tool