University of Pittsburgh researchers have developed an innovative substrate processing technology that combines micro-molding and fiber deposition to manipulate cell biology and induce local anisotropy in biomaterial surfaces. This hybrid technique, known as Hybrid Micro-Molding Electrodeposition Surface (HMES) processing, enables precise control over substrate topography at the mesoscopic scale (10-250µm), offering a transformative approach for enhancing cellular interactions in medical devices and tissue engineering applications. By replicating native tissue extracellular matrix (ECM) architectures, this technology holds promise for improving cell adhesion, proliferation, and differentiation, leading to superior outcomes in regenerative medicine.
Description
The interaction between biomaterials and cells is significantly influenced by the surface topography of the materials. This technology leverages micro-molding and fiber deposition methods to create complex, patterned substrates that mimic the natural ECM, thereby optimizing the mechanical support and biochemical environment necessary for cell growth and function. HMES processing involves the deposition of micro-fibers onto a customized meso-scale pattern, allowing for enhanced nutrient exchange, cell migration, and tissue formation. The resulting substrate can be tailored to match the specific mechanical and topological requirements of various biomedical applications, such as cardiovascular devices and tissue-engineered scaffolds.
Applications
- Tissue Engineering
- Cardiovascular Devices
- Medical Implants
Advantages
This technology offers unprecedented control over substrate topography and fiber alignment at both the meso and micro scales, enabling the creation of biomimetic surfaces that closely replicate the natural extracellular matrix. By combining the strengths of micro-molding and fiber deposition, this method enhances cell-substrate interactions, improves mechanical and structural anisotropy, and supports large-scale processing for clinical applications. The ability to precisely tune pore size, fiber diameter, and alignment makes this technology versatile and scalable, providing significant advantages in the development of advanced biomaterials and tissue-engineered constructs.
Invention Readiness
The technology has been demonstrated at the prototype level, with successful application in enhancing vascular smooth muscle cell proliferation on micro-patterned films and scaffolds. The process has been validated through in vitro studies, showing superior cellular responses on HMES-processed substrates compared to conventional methods.
IP Status
https://patents.google.com/patent/US20240016983A1
