This system integrates a standard microscope base featuring a 20x/0.4 objective with advanced illumination provided by a xenon lamp, and precision wavelength control via an acousto-optic tunable filter. Off-axis parabolic mirrors, achromatic lenses, and beam-splitting optics work in concert to expand and collimate the light beam for the formation of high-quality images. A primary sCMOS camera captures multi-wavelength data (450–650 nm) while a motorized XY stage enables controlled, multi-area acquisition. Specially designed slides with a reflective coating and laser-etched registration marks facilitate improved signal-to-noise ratios and accurate pre- and post-staining image alignment for quantitative phase analysis.
Description
The technology is differentiated by its comprehensive integration of precise optical components and advanced imaging protocols tailored to weakly scattering, unstained biological samples. Its ability to combine high-resolution spectral imaging with quantitative phase microscopy sets it apart from traditional methods. The inclusion of a registration system and meticulously engineered optics ensures minimal refractive index mismatches and enhanced imaging fidelity, making it uniquely capable of delivering detailed, multi-dimensional insights into sample structure and composition.
Applications
Digital pathology imaging
Unstained tissue diagnostics
Quantitative phase analysis
Preclinical drug screening
Advantages
Enhanced signal-to-noise ratio for weakly scattering, unstained biological samples.
Precise spectral imaging across the visible wavelength range (450–650 nm) for quantitative phase analysis.
Multi-area acquisition enabled by a motorized XY scanning stage for comprehensive sample evaluation.
Specialized slide design with laser-etched registration marks for accurate image alignment before and after staining.
Integration of advanced optical components and synchronized hardware for reliable, high-resolution imaging performance.
Invention Readiness
The second-generation SL-QPM system has been designed and tested, demonstrating its effectiveness in detecting nanoscale structural characteristics. The technology is ready for further development and integration into optical microscopy systems, with potential applications in both research and healthcare settings.
IP Status
Research Tool
