Avian mesenchymal stem cells are isolated, expanded, and characterized for their ability to differentiate into bone- and cartilage-forming cells. These cells are incorporated into biodegradable hydrogel scaffolds fabricated through advanced techniques such as photocrosslinking and 3D printing, using materials like methacrylated gelatin and hyaluronan. The scaffolds enable in situ cell differentiation when applied to skeletal defects, and their formation is triggered using visible light for precise, customizable repair. Efficacy is validated through a range of in vitro and ex vivo models with comprehensive histological, mechanical, biochemical, and molecular assessments.
Description
This technology stands apart by uniquely combining stem cell biology with sophisticated, light-activated hydrogel fabrication methods. Its integration of avian mesenchymal stem cells with customizable biomaterials creates a versatile platform suitable for addressing a diverse range of skeletal conditions, from fractures to congenital defects. Additionally, the inherent antibacterial properties of the cells add a critical layer of protection against infection. This dual approach of promoting tissue regeneration while mitigating infection risks offers a significant advancement over traditional repair methods, paving the way for more effective treatment options in avian skeletal medicine.
Applications
- Avian skeletal tissue engineering
- Customizable bone defect repair
- Antibacterial orthopedic treatment
- Veterinary regenerative medicine
- 3D printed bone scaffolds
Advantages
- Enables precise, customizable skeletal tissue repair through photopolymerizable hydrogel scaffolds and in situ crosslinking.
- Enhances regeneration by combining avian mesenchymal stem cells with advanced biomaterials for bone and cartilage formation.
- Reduces infection risks with demonstrated antibacterial properties against pathogens like Escherichia coli.
- Offers versatile treatment options across various avian species and skeletal defect types, including genetic conditions, fractures, and metabolic diseases.
- Provides robust validation using ex vivo models and comprehensive analysis methods (histological, mechanical, biochemical, and molecular).
IP Status
https://patents.google.com/patent/US10864234B2
