Related Technologies - University of Pittsburgh

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Models of DNA Mismatch Repair Deficiency

05460

The modified cells exhibit distinct growth patterns, apoptotic activity, and cytokine expression, as well as increased CD8+ T cell infiltration, providing a comprehensive platform to study how DNA repair defects can influence tumor dynamics and immune interactions.

This technology employs CRISPR-mediated inactivation of the MLH1 gene within CT26 and MC-38 murine colorectal tumor cell lines to simulate DNA mismatch repair deficiency, resulting in microsatellite instability.

- Provides mechanistic insights into how DNA mismatch repair deficiency leads to microsatellite instability and influences tumor growth.

Lin Zhang

Life Science Research Tool

2020-07-28

Models of DNA Mismatch Repair Deficiency (Including Panc02 and 4T1)

05503

These engineered cell lines allow researchers to compare tumors with intact and defective DNA repair mechanisms under identical conditions.

A CRISPR-based technology was developed to inactivate the MLH1 gene in four distinct murine cancer cell lines—CT26, MC-38, Panc02, and 4T1—yielding isogenic models that accurately mirror the microsatellite instability associated with DNA mismatch repair deficiencies.

This technology stands out due to its precise replication of microsatellite instability effects, enabling direct investigations into the role of DNA repair deficiency in tumor immunogenicity.

Lin Zhang

Life Science Research Tool

Oncology

2020-09-11

Novel Targeted Gene Therapy for Cancers

06452

Scientists from University of Pittsburgh have developed a novel approach to target cancer cells through the insertion of a “suicide gene” (HSV1-tk) into key gene fusion areas of the cancer genome.

• Creation of this unique breakpoint, present only in cancer tissue provides a target for therapeutic intervention which is less likely to result in drug resistance through gene mutation.

Scientists from University of Pittsburgh have developed a novel approach to target cancer cells through the insertion of a “suicide gene” (HSV1-tk) into key gene fusion areas of the cancer genome.

Jianhua Luo

Therapeutic Modality

Gene Therapy - CRISPR

Oncology

Prostate cancer Liver cancer

2023-07-06

T Cell Receptors Targeting Defective DNA Repair Proteins