Women's & Reproductive Health - University of Pittsburgh

Novel Immortal Antibody Secreting Cell Lines

Breast cancer

2022-09-14

06101

These patient-derived novel cell lines have the potential to identify therapeutic targets in ovarian cancer.

Using tissue from different high-grade serous epithelial ovarian cancer (EOC) patients, four unique antibody secreting plasma cell lines were isolated.

Anda Vlad

Transgenic Mice Expressing Human C19MC

Ovarian cancer

2022-07-13

06008

C19MCs are normally only expressed in the placenta during pregnancy, but abnormal expression has been associated with rare forms of cancers, particularly pediatric brain tumors.

Further research could build on this discovery to develop blood-based diagnostics of pregnancy complication related to placental dysfunction.

Yoel Sadovsky

A Pain-enabled in vitro System for Studying Pain Mechanisms and Developing Treatments

Obstetrics

2022-04-18

05994

Researchers at Pitt have developed a pain-enabled in vitro system to study pain mechanisms and develop treatments specifically related to osteoarthritis (OA) and its associated pain.

Hang Lin

Musculoskeletal Neuroscience

OsteoporosisPain

2022-03-28

Breast Cancer Patient Derived Organoids

05679

This technology involves a collection of patient-derived breast cancer models developed from freshly isolated tumor tissues obtained within 60 minutes post-surgery.

This approach is differentiated by its rapid tissue procurement process and meticulous adherence to protocols that capture the intrinsic complexity of breast cancer.

- Provides a robust preclinical model that offers enhanced phenotypic fidelity compared to conventional breast cancer cell lines.

Adrian Lee

Genetically Engineered MCF-7 Cells for pin1-driven Breast Cancer Cell Cycle Research

2021-04-28

05275

The technology involves a genetically engineered MCF-7 breast cancer cell line that has been modified to overexpress the Pin1 protein, a key peptidyl-prolyl cis/trans isomerase important for cell cycle regulation.

This cell model facilitates detailed investigations into the cellular mechanisms governing breast cancer progression and provides a platform for screening compounds that might affect cell cycle dynamics.

- Preclinical assay platform. - Target validation studies. - Breast cancer research tool. Advantages. - Provides a reliable in vitro model to study the role of Pin1 in breast cancer and cell cycle regulation.

Shivendra Singh

Genetically Defined Murine Ovarian Cancer Cell Lines for Advanced Research

Breast cancer

2020-03-11

03711

• Exploration of new therapeutic strategies for ovarian cancer.

Researchers at the University of Pittsburgh have developed a collection of novel murine ovarian cancer cell lines with defined genetic mutations.

Unlike existing models, these cell lines are derived from primary ovarian tumors, providing a more accurate representation of human ovarian cancer for preclinical studies and therapeutic development.

Anda Vlad

CAG-GFP Transgenic Mouse on a DBA/2 Genetic Background

Ovarian cancer

2015-09-01

03099

The innovators generated a transgenic DBA/2 mouse that expresses a reporter gene, enhanced green fluorescence protein (EGFP), ubiquitously.

Kyle Orwig

Male genitourinary

2013-07-29

VASA-GFP Transgenic Rat

03100

The innovators generated a transgenic rat that expresses a reporter gene, enhanced green fluorescence protein (EGFP), specifically in the germline of male and female rats under the control of the Vasa (Ddx4) gene promoter.

Kyle Orwig

Putative Cancer Stem Cells (CSCs) from Mouse Mammary Tumor

Male genitourinary

2013-07-29

02465

Dr. Khillan and colleagues have established a unique line of putative Cancer Stem Cells (CSCs) from a mouse mammary tumor which exhibit the typical properties of stem cells such as growth as multi cellular three dimensional colonies, absence of contact inhibition, alkaline phosphates staining and continuous self renewal capability.

Jaspal Khillan

Breast cancer

Women's Health

2011-04-19

Fetal Specific Knockout

01747

This disclosure involves the use of the cre/lox mouse knockout system to produce a fetal specific knockout in order to study the fetal signaling during pregnancy.

Jeyasuria Pancharatnam

Reagent Mouse for 15-Lipoxygenase-1 Enzyme Study Examines the Efficacy of the Therapeutic Compounds

Obstetrics

2008-05-23

00920

High 15-LO-1 activity is observed in multiple forms of cancers (prostate, skin, lung, pancreatic, colorectal and breast), as well as in diseases such as glomerulonephritis (kidney), atherosclerosis (heart), arthritis (bone), Alzheimer's (brain) and osteoporosis (bone).

The inventors have determined that altering/inhibiting the 15-LO-1 enzyme activity in tissues will help identify individuals that respond to therapeutic interventions (directed against specific diseases) ranging from abnormal cell growth (cancer/osteoporosis) and/or immune/inflammatory response (arthritis, kidney disease, infarction).

Uddhav Kelavkar